Table 1. Cell culture media composition and suppliers.

Medium	Abbreviation	Base	Supplements	Manufacturer
Keratinocyte serum free medium	KSFM	Serum free medium with L-glutamine	25 μg/mL bovine pituitary extract 1 ng/mL epidermal growth factor 50 U/mL penicillin 50 μg/mL streptomycin	Gibco, Thermo Fisher Scientific
Melanocyte growth medium	MGM	PC-1 base medium	2% PC-1 supplement 1% L-glutamine 5 ng/mL basic fibroblast growth factor 24.6 g/mL N6,2′-O-Dibutyryl-adenosine 3′,5′-cyclic monophosphate 50 U/mL penicillin 50 μg/mL streptomycin	Base: Lonza Supplements: Sigma-Aldrich
Fibroblast medium	FM	Dulbecco’s modified Eagle medium	10% foetal calf serum 50 U/mL penicillin 50 μg/mL streptomycin	Gibco, Thermo Fisher Scientific