Table 2. Viability measurements using (4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay of primary keratinocytes and melanocytes stained with 5(6) carboxyfluorescein-N-hydroxysuccinimidyl ester (CFSE).
Keratinocytes | ||||
CFSE | 24 hours | 48 hours | 72 hours | 7 days |
0 μM | 100.2±36.1 | 100.2±12.6 | 100.0±11.9 | 100.0±18.2 |
5 μM | 88.6±18.4 p = 0.720 | 99.83±24.7 p>0.999 | 100.0±12.9 p>0.999 | 100.0±19.9 p>0.999 |
10 μM | 100.2±22.0 p>0.999 | 100.0±18.7 p>0.999 | 88.2±12.1 p = 0.670 | 85.2±26.1 p = 0.484 |
Melanocytes | ||||
CFSE | 24 hours | 48 hours | 72 hours | 7 days |
0 μM | 78.5±15.2 | 101.0±5.6 | 99.8±14.9 | 99.8±17.4 |
5 μM | 97.5±16.0 p = 0.133 | 96.8±9.5 p>0.999 | 132.6±20.0 p = 0.004 | 114.0±19.0 p = 0.839 |
10 μM | 75.0±13.9 p>0.999 | 113.0± 15.6 p = 0.879 | 116.5±24.2 p = 0.230 | 107.2±9.5 p>0.999 |