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. 2019 Aug 29;14(8):e0221878. doi: 10.1371/journal.pone.0221878

Table 2. Viability measurements using (4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay of primary keratinocytes and melanocytes stained with 5(6) carboxyfluorescein-N-hydroxysuccinimidyl ester (CFSE).

All values reported as percent relative to unstained controls (at time 0) ± SD, with Bonferroni’s multiple comparisons test corrected p-values.

Keratinocytes
CFSE 24 hours 48 hours 72 hours 7 days
0 μM 100.2±36.1 100.2±12.6 100.0±11.9 100.0±18.2
5 μM 88.6±18.4 p = 0.720 99.83±24.7 p>0.999 100.0±12.9 p>0.999 100.0±19.9 p>0.999
10 μM 100.2±22.0 p>0.999 100.0±18.7 p>0.999 88.2±12.1 p = 0.670 85.2±26.1 p = 0.484
Melanocytes
CFSE 24 hours 48 hours 72 hours 7 days
0 μM 78.5±15.2 101.0±5.6 99.8±14.9 99.8±17.4
5 μM 97.5±16.0 p = 0.133 96.8±9.5 p>0.999 132.6±20.0 p = 0.004 114.0±19.0 p = 0.839
10 μM 75.0±13.9 p>0.999 113.0± 15.6 p = 0.879 116.5±24.2 p = 0.230 107.2±9.5 p>0.999