Fig 1. Coordinated intra-organ growth involves the upregulation of Dmp53 target genes.

(A) Representative adult wings from individuals expressing GFP, RA^CS or dmyc^RNAi under the control of ci-Gal4, which drives expression to the anterior compartment of wing imaginal discs (shown in light blue). Numbers indicate total wing area represented as a percentage of GFP-expressing wings. (B-C, G-H) Histograms plotting normalized area (B-C) or cell density values (G-H) of the anterior (A) and posterior (P) compartments of adult wings from individuals expressing RA^CS or dmyc^RNAi with the indicated Gal4 drivers. Blue bars indicate the transgene-expressing compartment and grey bars indicate the adjacent wild-type compartment. Expression of RA^CS or dmyc^RNAi significantly reduced the size of both transgene-expressing and non-expressing domains. Horizontal line shows the size or cell density values of the normalized control GFP-expressing wings. ** p<0.01. (D-F) BrdU incorporation assay in larval wing discs from individuals expressing GFP, along with the indicated transgenes under the control of ci-Gal4 or en-Gal4. (I) Transcriptome analysis of RNA samples obtained from the P compartment (GFP-positive cells) of wing imaginal discs from individuals expressing GFP or GFP plus RA^CS with en-Gal4. 179 genes were differentially expressed between RA^CS-expressing and wild-type cells (-1≥fc≥1; p≥0.05). (J) Gene ontology (GO) analysis showed highly enriched GO terms amongst genes upregulated in RA-expressing wing discs. -Log 10 of p-value are shown. GO-BP, biological processes. GO-MF, molecular function. GO-CC, cellular component.