(A) Representative adult wings from individuals expressing the indicated transgenes in posterior compartment (blue) under the control of en-Gal4. The expected size of the neighboring domain to give rise to a well-proportioned adult wing is shown. (B) Histogram plotting normalized area of the posterior (P; blue bars) and anterior (A; grey bars) compartments of adult wings from individuals expressing the indicated transgenes with en-Gal4. Expression of Dmp53RNAi, eigerRNAi or eigerIR reverted the non-autonomous reduction in tissue size caused by RA expression. ** p<0.01. (C-H) Wing discs expressing GFP along with RACS in the posterior compartment by using en-Gal4 in different genetic backgrounds (wild-type (C,F), co-expressing eigerRNAi (D,G) or eiger3/3 (E,H)) and labeled to visualize GFP (green), TUNEL (red in C-E; grey in C’-E’) and BrdU incorporation (red in F-H; grey in F’-H’).