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. Author manuscript; available in PMC: 2019 Aug 29.
Published in final edited form as: Free Radic Res. 2019 Feb 18;53(3):324–334. doi: 10.1080/10715762.2019.1575512

Figure 3.

Figure 3.

Celastrol systematically inhibits angiogenesis-related gene transcription in CRC. HT29 cells were treated with celastrol or vehicle control for 24 h. RNA was then extracted from cells and converted into complementary DNA using a cDNA synthesis kit. The cDNAs were then subjected to the angiogenesis PCR array. (A) A plot comparing the 2 μM celastrol-treated group with the control group. (B) A plot comparing the 3 μM celastrol-treated group with the control group. Among the 86 genes tested, most of the genes were down-regulated by celastrol treatment at the mRNA level. Yellow dots indicate up-regulated genes. Black dots indicate unchanged genes. Blue dots indicated down-regulated genes. (C) The heat map displays the gene expression pattern in the angiogenesis pathway of CRC cells treated with or without celastrol. CEL = celastrol.