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. 2019 Aug 19;129(9):3877–3893. doi: 10.1172/JCI123374

Figure 2. Increased FBXW7 expression in inflamed intestinal tissues in mice.

Figure 2

(A) Representative immunohistochemical staining of colon specimens obtained from control mice and mice with colitis treated for 5 days with DSS. Scale bars: 50 μm. Arrows point to macrophages with high expression of FBXW7. (B) Fbxw7 mRNA expression in CLP CD11b+CX3CR1+ macrophages from mice with DSS-induced colitis (day 9) and 12-week-old Il10–/– mice (n = 6). (C) Quantitative assessment of Fbxw7 mRNA expression in peripheral blood monocytes from mice with colitis at different time points after DSS challenge (n = 6). (D) Representative flow cytometric analysis of FBXW7 expression in murine Ly6ChiCX3CR1int colonic monocytes at steady and inflammatory states. FVD, Zombie Violet Fixable Viability Dye. Immunofluorescence staining for (E) FBXW7 (green), CD68 (red), and DAPI for nuclei (blue) and (F) FBXW7 (green), F4/80 (red), and DAPI for nuclei (blue) in colon tissues from control mice and mice with DSS-induced colitis. Scale bars: 100 μm (whole colon sections) and 50 μm (enlarged insets). Red arrows point to F4/80+ macrophages with high expression of FBXW7. *P < 0.05 and **P < 0.01, by unpaired, 2-tailed Student’s t test. Data are presented as the mean ± SD of at least 3 independent experiments.