Figure 3. LysM⁺Fbxw7^fl/fl mice show attenuated experimental colitis.

Fbxw7^fl/fl and LysM⁺ Fbxw7^fl/fl mice were administered water or 3% DSS for 7 days to induce acute colitis, followed by a 2-day recovery period on normal drinking water. (A) Body weight changes, diarrhea, and rectal bleeding scores were assessed daily as described in Methods (n = 5). (B) Gross morphology of colons from Fbxw7^fl/fl and LysM⁺ Fbxw7^fl/fl mice. Colon lengths were measured on day 9 (n ≥ 5). (C) H&E-stained images of colon sections. Scale bars: 200 μm (whole colon section) and 50 μm (enlarged insets). (D) Fbxw7^fl/fl and LysM⁺ Fbxw7^fl/fl mice were administered 4% DSS to induce acute colitis. Survival of mice was monitored until day 15 (n = 10). ***P < 0.001, by log-rank test. (E) Body weight changes during intestinal inflammation and the recovery period (n = 5). (F) Length of colons from Fbxw7^fl/fl and LysM⁺ Fbxw7^fl/fl mice sacrificed after 7 days of 3% DSS treatment and 8 days’ recovery with drinking water (n = 5). Data are presented as the mean ± SEM and are representative of 3 independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001, by unpaired, 2-tailed Student’s t test (A, B, E, and F).