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. 2019 Aug 5;129(9):3702–3716. doi: 10.1172/JCI93820

Figure 6. Enteric resident bacteria in mice increase the capacity of intestinal B cells to suppress ex vivo bacterial lysate–stimulated inflammatory cytokine production that is mediated by IL-10 signaling.

Figure 6

(A) Cytokine secretion by 10 μg/mL CBL-stimulated colonic LP B cells isolated from GF or SPF-raised WT mice in culture for 2 days. Cytokine concentrations were measured by ELISA. Data are presented as median of 6–7 separate cell cultures, with cells in each culture pooled from 3–4 mice. Mann-Whitney U test (GF vs. SPF). *P < 0.05, **P < 0.01, ***P < 0.001. (B) Colonic B cells from GF or SPF-reared WT mice were cultured with anti–IL-10R or isotype control antibodies in the presence or absence of 10 μg/mL CBL for 24 hours. Supernatant levels of IL-12p40 were measured by ELISA. Data are presented as median of 4 separate cell cultures, with cells in each culture pooled from 2–4 mice; *P < 0.05, Kruskal-Wallis test with Dunn’s post hoc test. (C) Cytokine secretion by MLN B cells from GF or SPF-raised WT mice cultured for 2 days with the indicated bacteria lysates or TLR ligands (see Figure 5 legend). IL-10 and IL-12p40 in culture supernatants were measured by ELISA. Data are presented as median of 4 separate cell cultures, with cells in each culture pooled from 3–6 mice. *P < 0.05, **P < 0.01, ***P < 0.001 (vs. no stimulation), Kruskal-Wallis test with Dunn’s post hoc test; #P < 0.05, ##P < 0.01 (GF vs. SPF), Mann-Whitney U test.