Skip to main content
. 2019 Aug 5;129(9):3670–3685. doi: 10.1172/JCI123700

Figure 5. 4F treatment improves the inflammatory mediator profile of mouse macrophages.

Figure 5

*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. (A–C) LPS-activated (25 ng/mL) BMDMs from FLOX and MKO mice were cotreated with D-4F (15 mg/mL) for 24 hours (n = 3/group). 4F significantly suppressed Il1b (A) and Tnf (B) expression in both FLOX and MKO BMDMs (fold change vs. 0 hours). LPS significantly increased prostanoid production in FLOX but not MKO mice, whereas 4F significantly reduced the levels of PGE2 (C) and PGD2 (D) in FLOX lysate from 10 hours. In FLOX lysate, 4F also selectively suppressed the COX pathway marker 11HETE without reducing the lipoxygenase product 15HETE (E). Statistical analyses were conducted as follows: (A, B) For each of the FLOX or MKO mice with or without 4F, 2-way ANOVA with Tukey’s multiple comparisons test and adjusted P values were used. (C, D) Three-way ANOVA with Tukey’s multiple comparisons test and adjusted P values were used. (E) For each of the 2 analytes, 2-way ANOVA with Tukey’s multiple comparisons test and adjusted P values were used.