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. 2019 Aug 19;129(9):3941–3951. doi: 10.1172/JCI127511

Figure 6. Dps does not activate GPCRs.

Figure 6

PRESTO-Tango GPCR assay system consists of 315 constructs in individual plasmids fused to a cleavable tTA transcription factor. These plasmids were transfected into 293T cell–derived cell line HTLA, which expresses a β-arrestin2-TEV fusion gene and tTA-induced luciferase reporter. When a specific GPCR is activated, the β-arrestin2-TEV fusion gene is recruited to the membrane-bound GPCR, where it cleaves tTA, allowing for its transport to the nucleus and subsequent activation of the tTA-activated luciferase reporter gene. Using a substrate to quantify luciferase expression, the activation of GPCRs by agonists was measured (median values of quadruplicates; 2 independent experiments). As a reference, activation of various GPCRs induced by kidney tissue lysates is shown in the separate columns.