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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Neuroendocrinol. 2019 Jul 15;31(8):e12762. doi: 10.1111/jne.12762

Fig 4.

Fig 4

Layer-specific effects of gonadal hormones and stress on microglial density and morphology in prelimbic cortex in male rats. A. Total microglial density based on estimated volume. B. Total area of Iba-1+ material. The area of Iba-1+ material was greater in CAST males, and was reduced in layers 2/3 and 5/6 in CAST+E males compared to SHAM animals. C. Process complexity per microglia. Microglial complexity was increased in layers 2/3 and 5/6 in CAST males, and decreased in CAST+E as compared to SHAM males. Stress decreased microglial complexity in CAST males, and increased microglial complexity in CAST+T males. CAST: Gonadectomized males without hormone treatment. +T: Treated with testosterone. +E: Treated with estradiol. †p < .05 compared to same layer SHAM group. †p < .05, v p < .10 compared to same layer SHAM group. *p < .05, ^ p < .10 compared to same hormone condition unstressed group. X-axis labels represent layer (1, 2/3, 5/6). Error bars indicate SEM. Number of animals per group: SHAM male (unstressed: 6, stressed: 6), CAST (unstressed: 6–8, stressed: 6–9), CAST+T (unstressed: 6, stressed: 5), CAST+E (unstressed: 6, stressed: 7). CAST outlier removed from microglial complexity analysis (unstressed: layer 2/3, n = 1).