Figure 6.

Cigarette sidestream smoke enhances ROS production via formation of TRPC3‐Nox2 protein complex in cardiomyocytes. (a) DHE‐positive ROS production in H9c2 cells. H9c2 cells were treated with cigarette sidestream smoke‐containing medium (CSM, 5%), LPS (1 μg·ml⁻¹), high glucose (HG, 40 mM), and methyl mercury (MeHg, 1 μM) for 24 hr (n = 5). Scale bar: 50 μm. (b, c) Effects of ibudilast and Pyr3 on CSM‐induced ROS production (b) and Nox2 up‐regulation (c) in H9c2 cells (n = 5). Scale bar: 50 μm. Cells were treated with the ibudilast (10 μM) and Pyr3 (3 μM) 30 min prior to CSM treatment (5% for 24 hr, n = 5). Data are shown as the mean ± SEM. *P < .05, significantly different as indicated; one‐way ANOVA with Tukey's comparison test