Figure 2.

Different modes of delivering AAV for CNS gene expression in mice. (a) Common methods reported for delivering AAV‐mediated gene expression in neonatal mice include (A) direct stereotaxic brain infusion, (B) spinal cord injections, and (C) intravascular delivery. (b) Techniques most frequently reported for delivering AAV‐mediated gene expression in mature mice include (D) direct stereotaxic brain infusion, (E) spinal cord injections, (F) intravascular, and (G) i.m. delivery. (c) Two commonly used methods for intracerebral delivery of AAV in adult brains are (H) intracerebrovascular injection for widespread gene expression or (I) into distinct brain areas for localized expression. Both techniques require the use of stereotaxic devices for guided injections