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. 2019 Aug 29;9:12580. doi: 10.1038/s41598-019-48884-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of cMLCK proteins in mouse and human hearts. Representative co-immunostaining images of cMLCK, MLC2v and α-actinin2 using hearts from wild-type (A,B) or conditional Mylk3 knockout mice (C). Co-immunostaining of cMLCK and α-actinin2 in wild-type mouse (D) and human hearts without apparent disease (E). (F) Co-immunostaining of cMLCK and α-actinin2 (left panel) and cMLCK and M-line protein myomesin (right panel) in isolated mouse adult cardiomyocytes. Bars = 10 µm in A, B, D, E; 20 µm in C; 100 or 20 µm in F.