Figure 5.

Effect of cMLCK mutations in adult cardiomyocytes. (A) Representative beta gal-stained heart 1 week after a single injection of adenovirus-lacZ (1 × 10¹¹ viral particle in 30 µl) into the anterior LV wall. (B) Time schedule of experiments. (C) Representative Western blotting with HA and GAPDH antibodies using whole heart lysates at 2, 4 and 7 days after injection of adenovirus. (D) Representative adult cardiomyocytes isolated from Mylk3^flox/flox/Cre mice infected with three viral constructs co-immunostained with HA (green) and α-actinin2 (red). Enlarged images were shown at the bottom. See Supplemental Fig. 3 for additional immunostaining images. (E) Summary of cellular area (µm²), long-axis (µm), and short-axis (µm). Number of cardiomyocytes measured was indicated on graph. Mean ± S.E.M. Results were compared using one-way ANOVA followed by post-hoc test (SPSS ver. 25). *P < 0.05.