Fig. 4.

Nucleotide-independent oligomerization. a The molecular masses of IniA (theoretical molecular mass 65.9 kDa) were determined from sedimentation coefficients measured by analytical ultracentrifugation in the absence or presence of 2 mM GDP/GMPPNP/GTP or 2 mM GDP, 5 mM AlCl₃,10 mM NaF. The data are representative of at least three repetitions. b Liposomes (78 mol% PE, 12 mol% PG, 6 mol% CL, 4 mol% PA, final lipid concentration 2 mM) were mixed in various conditions, 2 μM IniA was added at initiation, and the absorbance was measured at 405 nm. The data are representative of at least three repetitions. c As shown in (b), the results were observed using a fluorescence microscope. Scale bar, 40 µm. d As shown in (b), 2 mM Mg²⁺ and 5 mM GTP (or 5 mM ATP as a control) were added after a period of reaction. The source data of Figs. 4a, b and d are provided in the Source Data file