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. 2019 Aug 29;9:12496. doi: 10.1038/s41598-019-48961-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitotic spindle and centromer formation in T. gondii-infected BUVEC. (A) T. gondii-infected BUVEC and control cells were stained for chromosomes by DAPI (blue), for mitotic spindles by α-Tubulin (red) and for centromeres by γ-Tubulin (green) and analyzed via confocal microscopy. Additionally, the intensity of centromer-related signals was assessed and plotted as a graph showing intensity value vs distance (in µm). Scale bar represents 5 µm. (B) The number of centrosomes in mitotic cells, in T.gondii-infected BUVEC and control cells, were counted, and graph as a percentage of the total mitotic cells in the monolayer. Bars represent the median ± SEM. Bars represent the median ± SEM. Nonparametric one-way ANOVA (Kruskal-Wallis post-test), **p ≤ 0.001.