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. 2019 Aug 29;9:12534. doi: 10.1038/s41598-019-47609-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular protein of interleukin 1 beta (IL-1β) and inactive caspase-1 responses from peripheral blood mononuclear cells (PBMCs) were measured by Western blotting after stimulation with dead Mycobacterium avium complex (dMAC) or infection by viable MAC (MAC) bacilli (multiplicity of infection: 100) for 24 hours. (A) Case demonstration and (B–D) bar charts for the protein levels of pro-caspase-1, pro-IL-1β and mature IL-1β by standardizing using individual internal control (GAPDH) and negative control. The data were compared using the Mann Whitney U test. The crossed lines are median values and bars of inter-quartile range. HC, healthy controls; LD, lung disease; NC, negative control. Full-length blot is presented in Supplementary Fig. S3.