Fig. 3.

GSNOR is required for root tolerance to high Fe. a Seedlings of gsnor knockout mutants (hot5-2 and hot5-4) and the respective wild-type (Col-0 and Ws-4) upon high Fe (250 µM) treatment for 6 days. Scale bar, 5 mm. b Mean root length of wild-type (Col-0), gsnor mutant (hot5-2), complemented line (GSNOR-GFP), and over-expression (GSNOR-OE) line. Error bars: standard deviation. n = 15 biologically independent samples. Asterisk * and ** represent the significant differences between high Fe (150 µM Fe in blue asterisk, 250 µM Fe in red asterisk) and the control as determined by Student’s t-test at p-value < 0.05 and 0.01, respectively. The source data of Fig. 3b are provided in a Source Data file