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. 2019 Aug 29;9:12514. doi: 10.1038/s41598-019-48973-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative Western blot images of p62 and autophagy-related proteins (Atg) 3, 7 and 12, and microtubule-associated protein 1 light chain 3 (LC3), beclin-1, class III phosphatidylinositol 3-kinase (VPS34) and UV radiation resistance-associated gene protein (UVRAG). Samples were obtained from SHRSP5/Dmcr rats fed control and HFC diets for 2 and 8 weeks. (B) Data are presented as means (bar) and dot plots for individual data. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. Wide-length blots/gel image are presented in Supplementary information. Data was analysed by two-way analysis of variance between diet and feeding duration (week). P values of diet, and week, D × W (diet × week interaction effect) were expressed within each graph. *Significant difference between HFC diet and control-diet groups, P < 0.05.