Table 1.
Different N-glycan structures and relative intensities for the native and two recombinant preparations of G-hPRL
| N-Glycan | Underivatized mass (–H2O) (Da) | Relative intensity of each N-glycan per each preparation (%) | |||
|---|---|---|---|---|---|
| HEK-G-hPRL | CHO-G-hPRL | Pit-G-hPRL | |||
| 1 | 0 | 892.3 | – | – | 3.9 |
| 2 | F1 | 1038.4 | – | – | 8.2 |
| 3 | M1 | 1054.4 | – | – | 0.4 |
| 4 | M2 | 1216.4 | 9.0 | 4.6 | 0.7 |
| 5 | N1F1 | 1241.5 | 5.8 | – | 0.3 |
| 6 | N2F1(-M) | 1282.5 | 4.5 | – | – |
| 7 | M2P1 | 1296.4 | – | – | 1.1 |
| 8 | M3 | 1378.5 | – | 1.0 | 0.5 |
| 9 | N1G1F1 | 1403.5 | – | – | 0.4 |
| 10 | N2F1 | 1444.5 | 11.0 | – | 0.5 |
| 11 | M3P1 | 1458.5 | – | 7.6 | 3.1 |
| 12 | N3 | 1501.6 | 3.5 | – | – |
| 13 | N1G1M2 | 1581.6 | 3.0 | – | – |
| 14 | N2F2 | 1590.6 | – | – | 0.5 |
| 15 | N2G1F1 | 1606.6 | 4.1 | 1.3 | 0.5 |
| 16 | N2G2 | 1622.6 | 2.7 | – | – |
| 17 | N3F1/N2Gn1F1 | 1647.6 | 9.4 | – | 0.6 |
| 18 | N3G1 | 1663.6 | 2.9 | – | – |
| 19 | M1N1Gn1F1(SO4)1 | 1686.6 | – | – | 3.2 |
| 20 | N1G1S1F1 | 1694.6 | – | 0.9 | 0.6 |
| 21 | M1N1G1S1 | 1710.6 | – | 1.1 | – |
| 22 | N2Gn1F1(SO4)1 | 1727.6 | – | – | 1.8 |
| 23 | N2G1F2 | 1752.7 | 2.6 | – | 0.8 |
| 24 | N2G2F1 | 1768.6 | 2.4 | 5.0 | – |
| 25 | N2G3 | 1784.6 | 3.3 | – | – |
| 26 | N2Gn1F2 | 1793.7 | – | – | 1.4 |
| 27 | N3G1F1 | 1809.7 | 4.2 | – | – |
| 28 | N1S2F1 | 1823.6 | 2.9 | – | – |
| 29 | N4F1 | 1850.7 | 4.3 | – | – |
| 30 | N2G1Gn1F1(SO4)1 | 1889.6 | – | – | 1.3 |
| 31 | N2G1S1F1 | 1897.7 | – | 0.6 | – |
| 32 | N2G2S1 | 1913,7 | 1.8 | 1.8 | – |
| 33 | N2G2F2 | 1914.7 | 2.1 | – | 0.5 |
| 34 | N2Gn2F1(SO4)1 | 1930.6 | – | – | 4.1 |
| 35 | N2G4 | 1946.7 | 1.6 | – | – |
| 36 | N3G1F2 | 1955.7 | 2.7 | – | – |
| 37 | N1S2F2 | 1969.7 | 2.7 | – | – |
| 38 | N3G2F1 | 1971.7 | 1.8 | – | – |
| 39 | N3G3 | 1987.7 | 1.6 | – | – |
| 40 | N4F2 | 1996.7 | 3.2 | – | – |
| 41 | N2Gn2F2 | 1996.8 | – | – | 0.6 |
| 42 | N2Gn2F1(SO4)2 | 2010.6 | – | – | 28.1 |
| 43 | N4G1F1 | 2012.8 | 1.7 | – | – |
| 44 | N2G1Gn1F2(SO4)1 | 2035.7 | – | – | 1.7 |
| 45 | N2G2S1F1 | 2059.7 | – | 12.0 | 0.5 |
| 46 | N2G2F3 | 2060.8 | 1.4 | – | – |
| 47 | N2G3S1 | 2075.7 | 1.6 | – | – |
| 48 | N2Gn2F2(SO4)1 | 2076.7 | – | – | 33.8 |
| 49 | N3G2S1 | 2116.8 | 2.4 | – | – |
| 50 | N2Gn2F3 | 2142.8 | – | – | 0.5 |
| 51 | N2G2S2 | 2204.8 | – | 6.9 | – |
| 52 | N2G2S2F1 | 2350.8 | – | 56.1 | 0.6 |
| 53 | N3G3S2F1 | 2715,9 | – | 0.8 | – |
| 54 | N3G3S3 | 2861.0 | – | 0.3 | – |
| Number of N-glycan structures | n = 28 | n = 14 | n = 28 | ||
| Fucosylated glycans (%) | 66.8 | 76.7 | 90.5 | ||
| Sialylated glycans (%) | 11.4 | 80.5 | 1.7 | ||
| Sulfated glycans (%) | – | – | 74.0 | ||
Abbreviations for N-glycans were made by not considering the basic pentasaccharide nucleus (“zero”) and adding all other monosaccharides, as stated in Table 1 and Fig. 6, in the following order: Man (M); GlcNAc (N); Gal (G); GalNAc (Gn); NeuAc/sialic acid (S); Fuc (F). So, for example, NeuAc1 Gal1 GlcNAc2 Fuc1 + Man3 GlcNAc2, becomes N2G1S1F1 (Capone et al. 2015)