Table 2.

Molecular mass of HEK cell-derived glycosylated prolactin obtained via N-glycoprofiling analysis and compared to MALDI–TOF-MS determination

Via N-glycoprofiling			Via MALDI–TOF-MS
Average N-glycan mass (Da)	G-hPRL MM (Da)a	Carbohydrate moiety (%)b	NG-hPRL (Mr)	G-hPRL (Mr)	Carbohydrate moiety (%)	Difference between G-hPRL MM and Mr (%)
1656.3	24,554.1	6.7	23,035.9	25,123.5	8.3	− 2.27

^aCalculated by adding the average N-glycan mass to the calculated NG-hPRL mass of 22,897.75 (Capone et al. 2015)

^bCalculated as a percent of the average glycan mass on G-hPRL MM