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. 2019 Aug 29;9:12520. doi: 10.1038/s41598-019-48970-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression of archetypic housekeeping genes in MCF7 cells and MEFs exposed to GO. In MCF7 cells, (a) ACTB and (b) GAPDH expression were normalized to the geometric mean of GAPDH and HMBS Cqs. In MEFs, (c) Actb and (d) Gapdh mRNA levels were normalized to the geometric mean of Rpl30 and Tbp. The combination of two reference genes was selected according to NormFinder results. Bars represent fold change; error bars represent propagation of standard error (SE). *p < 0.05 and **p < 0.01, assessed by one-way ANOVA and Tukey’s test, n = 3.