Table 3.
Gene | geoMean [Cq] | SD [±Cq] | CV [%Cq] |
---|---|---|---|
RPS13 | 17.70 | 0.25 | 1.42 |
RPL27 | 17.51 | 0.41 | 2.37 |
RPL30 | 21.66 | 0.30 | 1.39 |
OAZ1 | 20.04 | 0.27 | 1.34 |
ACTB | 14.02 | 0.58 | 4.15 |
GAPDH | 15.48 | 0.17 | 1.07 |
MAPK1 | 20.07 | 0.19 | 0.95 |
UBC | 24.09 | 0.18 | 0.75 |
HMBS | 19.33 | 0.19 | 1.00 |
TBP | 21.28 | 0.22 | 1.05 |
Geometric mean (geoMean), standard deviation (SD) and coefficient of variance (CV) of Cq data from ten candidate reference genes in MCF7 cells exposed to increasing concentrations of GO (n = 12). CV is calculated as the percentage of the Cq SD to the mean Cq.