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. 2019 Aug 29;9:12569. doi: 10.1038/s41598-019-48991-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of Oga in hematopoietic stem cells. (a) OGA activity was quantified with an established in vitro fluorescence assay²¹ using lysates from wildtype (WT) or Oga^Vav-Cre bone marrow, N = 3, error bars represent standard deviation. (b) O-GlcNAc was assessed by western blot using lysates from liver and bone marrow of WT and Oga^Vav-Cre (Vav-Cre) mice using RL2 for O-GlcNAc and β-Actin as loading control. Lysates from liver and bone marrow were run out on separate gels and transferred to nitrocellulose membranes (full length blots can be found in Supplemental Fig. S7) that were cut at 55 kDa to allow for simultaneous quantitation of O-GlcNAc and β-Actin. (c) Quantitation of western blot from panel b with O-GlcNAc intensity normalized to β-Actin intensity, with error bars representing range. Black bars represent wildtype and blue bars represent Oga^Vav-Cre.