Figure 2.

Reduced bone marrow progenitor populations in Oga^Vav-Cre mice. (a) Quantitation of total number of bone marrow cells from the indicated genotype. (b) Representative flow cytometric analysis, with boxes depicting gating, of long-term hematopoietic stem cells (LT-HSC, Lin⁻Sca1⁺Kit⁺ CD150⁺Flt3⁻), short-term hematopoietic stem cells (ST-HSC, Lin⁻Sca1⁺Kit⁺ CD150⁻Flt3⁻), multipotent progenitors (MPP, Lin⁻Sca1⁺Kit⁺ CD150⁻Flt3⁺) and lymphoid primed MPP (LMPP, Lin⁻Sca1⁺Kit⁺ CD150⁻Flt3⁺), common lymphoid progenitors (CLP, Lin⁻Kit^intFlt3⁺CD127⁺), B cell-biased lymphoid progenitors (BLP, Lin⁻Kit^intFlt3⁺CD127⁺Ly6d+) granulocyte/macrophage progenitors (GMP, Lin⁻Kit⁺Sca⁻CD16/32⁺CD34⁺) and common myeloid progenitors (CMP, Lin⁻Kit⁺Sca⁻CD16/32⁻CD34⁺). (c) Graphs depicting the absolute numbers of the indicated cell populations for the indicated genotype. Black bars represent wildtype and blue bars represent Oga^Vav-Cre. N = 4–6, error bars represent standard deviation, *p < 0.05, **p < 0.01 as determined by t-test.