FIG 2.

Physical and functional map of pLPV1Z (A), pLPV2Z (B), and pLPV3Z (C) promoter-probe vectors. The nucleotide sequence upstream of the reporter gene is shown for each vector. In all vectors, the ribosome binding site (RBS) is provided by the canonical sequence 5′-AAAGAGGAGAAA-3′, preceded by stop codons in the three reading frames (red). Unique cutter restriction enzymes are indicated in bold. Nomenclature: ble, Zeo resistance gene; aacC1, Gm resistance gene; ori ColE1-like, ColE1-derived origin of replication for E. coli; oriAb, origin of replication for Acinetobacter spp. All genes are reported in scale over the total length of the vector. Images were obtained by Snapgene software (GSL Biotech).