. 2019 Aug 29;85(18):e01334-19. doi: 10.1128/AEM.01334-19

TABLE 2.

Bacterial strains and plasmids

Strain or plasmid	Relevant characteristic(s)^a	Source and/or reference
Strains
A. baumannii
ATCC 19606T	Clinical isolate, type strain, antibiotic susceptible	67
ATCC 17978	Clinical isolate, antibiotic susceptible	39
ACICU	MDR clinical isolate, prototype of the international clonal lineage II	68
AYE	MDR clinical isolate, prototype of the international clonal lineage I	31
A. baylyi BD413	Naturally transformable strain	69, 70
A. dijkshoorniae 271	Member of the ACB complex	Seifert collection (71)
A. nosocomialis UKK_0361	Member of the ACB complex	Seifert collection (22)
A. pittii UKK_0145	Member of the ACB complex	Seifert collection (22)
A. seifertii HS A23-2	Member of the ACB complex	Seifert collection (72)
E. coli
DH5α	λ⁻ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(r_K⁻ m_K⁻) supE44 thi-1 gyrA relA1 Nal^r	63
H1717	fhuF::λplacMu aroB araD139 ΔlacU169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Km^r	44
Plasmids
pBluescript-II KS	E. coli cloning vector (GenBank accession number X52327.1), Ap^r	73
mini-CTX-lux	Integration-proficient Pseudomonas aeruginosa vector for chromosomal lux gene fusions, source of lux operon, Tc^r	30
pVRL1	E. coli-Acinetobacter species shuttle cloning vector, source of ColE1-like and oriAb origins of replication, aacC1 and TA cassette, MCS, Gm^r	22
pVRL2lacZ	E. coli-Acinetobacter species shuttle plasmid for arabinose-regulated lacZ expression, source of lacZ reporter gene, Gm^r	22
pVRL2gfp	E. coli-Acinetobacter species shuttle plasmid for arabinose-regulated GFP expression, source of GFP reporter gene, Gm^r	22
pVRL1Z	pVRL1-derived vector carrying the ble gene, source of ble cassette, Zeo^r	22
pMP220	Broad-host range, low copy number promoter-probe vector based on the lacZ reporter gene, Tc^r	18
pMP220::PbasA	A. baumannii PbasA promoter cloned into pMP220, Tc^r	19
pBS::PbasA	A. baumannii PbasA promoter cloned into pBluescript-II KS (pBS), Ap^r	19
pWH1277	Full length pWH1277 ligated to pBluescript-II KS plasmid, Ap^r	This work
pWH1277-Δ1	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ2	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ3	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ4	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ5	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ6	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ7	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ8	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ9	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ10	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pWH1277-Δ11	Deletion derivative of pWH1277 cloned into pBluescript-II KS, Ap^r	This work
pAb	E. coli-Acinetobacter species shuttle plasmid employed as scaffold vector for pLPV promoter-probe plasmid construction, Gm^r	This work
pAbΔlac	pAb plasmid carrying a deletion of CAP binding site, lac promoter (Plac) and the first 24 codons of lacZα, Gm^r	This work
pLPV1	lux-based promoter-probe plasmid, Gm^r	This work
pLPV2	lacZ-based promoter-probe plasmid, Gm^r	This work
pLPV3	GFP-based promoter-probe plasmid, Gm^r	This work
pLPV1Z	pLPV1-derived vector carrying the ble cassette, Gm^r Zeo^r	This work
pLPV2Z	pLPV2-derived vector carrying the ble cassette, Gm^r Zeo^r	This work
pLPV3Z	pLPV3-derived vector carrying the ble cassette, Gm^r Zeo^r	This work
pLPV1Z::PuvrA	PuvrA promoter cloned in pLPV1Z, Gm^r Zeo^r	This work
pLPV2Z::PuvrA	PuvrA promoter cloned in pLPV2Z, Gm^r Zeo^r	This work
pLPV3Z::PuvrA	PuvrA promoter cloned in pLPV3Z, Gm^r Zeo^r	This work
pLPV1Z::PadhP	PadhP promoter cloned in pLPV1Z, Gm^r Zeo^r	This work
pLPV1Z::PyahK	PyahK promoter cloned in pLPV1Z, Gm^r Zeo^r	This work
pLPV1Z::PbasA	PbasA promoter cloned in pLPV1Z, Gm^r Zeo^r	This work
pLPV2Z::PbasA	PbasA promoter cloned in pLPV2Z, Gm^r Zeo^r	This work
pLPV3Z::PbasA	PbasA promoter cloned in pLPV3Z, Gm^r Zeo^r	This work

Nal^r, nalidixic acid resistant; Km^r, kanamycin resistant; Ap^r, ampicillin resistant; Tc^r tetracycline resistant; Gm^r, gentamicin resistant; Zeo^r, zeocin resistant; ACB, Acinetobacter calcoaceticus-Acinetobacter baumannii.