FIG 3.

Fractionation of STYM1 cellular extracts. (A) Trace of the absorbance at 280 nm of the eluate of gel filtration of pooled inhibitory fractions from anion-exchange chromatography. Fractions were collected following the void starting at 10 ml in 500-μl volumes. (B) Agar overlay of gel filtration fractions 7 through 12 from panel A. Fractions were spotted onto plates, allowed to dry under aerobic conditions, and overlaid with a soft top agar inoculated with P. gingivalis. Plates were imaged 2 days after anaerobic growth. (C) Coomassie stain of gel filtration fractions 7 through 13 after separation by SDS-PAGE. The 70-kDa band from fraction 9 was excised for mass spectrometry analysis.