U2OS cell lines used in the in vivo DNA repair assay.
A, A schematic representation of SETMAR (pRC1702 and pRC1704), SET (pRC1705) and MAR (pRC1706) and the locations of the different mutations (pRC1707 to pRC1710). B, A western blot for the Flag-tagged SETMAR in the U2OS, SM, SETF, MARF and SMF cell lines. The western blot was performed with anti-Hsmar1 transposase (SETMAR), anti-Flag (SETMAR-Flag, SET / MAR-Flag) and anti-β-tubulin antibodies. The cell lines are described in Table 2. U2OS, U2OSF: parental lines; SM: SETMAR; SMF: SETMAR-Flag; SETF: SET domain-Flag; MARF: MAR domain-Flag.