Table 2.
U2OS cell lines used in the in vivo DNA repair assay.
| Full name | Abbreviation | Expression level | Comparison to SMF3 cell line on western blots |
|---|---|---|---|
| T-Rex-U2OS-TO (parental line) | U2OS | Endogenous level | / |
| T-Rex-U2OS-SETMAR | SM2 | High | Visible with anti-Hsmar1 transposase antibody, not SMF3 |
| SM3 | Very high | Visible with anti-Hsmar1 transposase antibody, not SMF3 | |
| T-Rex-U2OS-TO-Flag (parental line) | U2OS-F | Endogenous level | / |
| T-Rex-U2OS-SET-Flag | SETF1 | Low | Lower than SMF3 with anti-Flag antibody |
| SETF2 | Medium/High | Higher than SMF3 with anti-Flag antibody | |
| T-Rex-U2OS-SET-N210A- Flag | SETF-N210A | Low | Lower than SMF3 with anti-Flag antibody |
| T-Rex-U2OS-MAR-Flag | MARF | Medium/High | Higher than SMF3 with anti-Flag antibody |
| T-Rex-U2OS-SETMAR-Flag | SMF1 | Medium | Similar to SMF3 with anti-Flag antibody |
| SMF2 | Medium | Similar to SMF3 with anti-Flag antibody | |
| SMF3 | Medium | / | |
| T-Rex-U2OS-SETMAR-N210A-Flag | SMF-N210A | Medium | Similar to SMF3 with anti-Flag antibody |
| T-Rex-U2OS-SETMAR R432A-Flag | SMF-R432A | Low | Lower than SMF3 with anti-Flag antibody |
| T-Rex-U2OS-SETMAR D483A-Flag | SMF-D483A | Low/Medium | Lower than SMF3 with anti-Flag antibody |