Figure 5.

GlyR β Subunits Restore GABA_ARs Functioning by Interrupting the Interaction between GABA_ARα₁ and GlyRα₁ Subunits

(A) Average glycine I_max values in HEK-293 cells co-expressing GABA_ARs (α₁β₂γ₂) and either homomeric or heteromeric hyperekplexic mutant α₁/β GlyRs.

(B) Average GABA I_max values in HEK-293 cells co-expressing GABA_ARs (α₁β₂γ₂) and either homomeric or heteromeric hyperekplexic mutant α₁/β GlyRs.

(C) GlyRα₁ was purified using GABA_AR α₁ antibodies in HEK-293 cells co-expressing GABA_ARs (α₁β₂γ₂) and homomeric or heteromeric hyperekplexic mutant α₁/β GlyRs, and the co-precipitating proteins were detected by immunoblotting. Inputs are immunoblots of the same protein in cell lysates before Co-IP. Quantification of WT and mutant GlyRα₁ binding to GABA_AR α₁ (n = 3). Data were normalized to the WT group.

All digits within the columns represent numbers of cells measured. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 based on unpaired t tests; ns, not significant (p > 0.05).