Figure 1.

Phospho-p65 (s311) Is Located in the Membrane of Dorsal Root Ganglion Neurons and Is Increased in Neuropathic Conditions

(A–C) The representative confocal images show that p-p65 is located not only in the nucleus but also in the membrane of DRG neurons in naive (A), vincristine (VCR)-treated (B) and L5-spinal nerve ligation (L5-SNL) (C) rats. Scale bars: left 100 μm, right 20 μm.

(D and E) The western blots show that p-p65 in membrane is increased in VCR-treated (D) and L5-SNL (E) rats, compared with vehicle and sham rats. Samples were harvested after the last VCR injection or 10 days after L5-SNL. n = 6 in each group. ∗∗p < 0.01, ∗∗∗p < 0.001 compared with vehicle or sham group.

(F) The cell types that express p-p65 (s311) in DRG neurons. Scale bars: 50 μm.

(G and H) The total (G) and the average (H) gray value of p-p65 signal in NF-200 positive (larger-diameter) and NF-200-negative (small-diameter) neurons. n = 40 in NF200-negative group, n = 62 in NF200-positive group. ∗∗∗p < 0.001 compared with the corresponding NF200-negative group. Two-tailed t test. The specificity of the antibody for p-p65 (s311) was identified in Figure S2A. Data expressed as mean ± SD. The control of membrane fractionation process was shown in Figure S2C.