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. 2019 Aug 23;10:1051. doi: 10.3389/fphys.2019.01051

FIGURE 2.

FIGURE 2

Relative peak stress development by isometrically contracting mantle muscle strips from cuttlefish (Sepia officinalis). Preparations were initially stimulated to contract six times at 0.2 Hz. Stress developed during this period served as the level at which all further challenges were related. All experiments involved paired preparations. Bathing media contained glucose (open bars) plus additional 25 μM CHX (stippled bars; left panels) or additional 1 mM IAA (stippled bars; right panels). Panels (A,B) – Δ stress development following a 10 min rest followed by a second wave of six contractions at 0.2 Hz. Panels (C,D) – Δ stress development following contractions for 30 s at 30 Hz [immediately following (A,B)]. Panels (E,F) – Δ stress development following 1 h rest [following (C,D)]. ∗Significant difference in response of matched preparation in media containing glucose alone relative to preparations in media with either additional CHX or IAA. N = 6 for CHX experiments and N = 5 for IAA experiments. Box and whisker plots show the range and median value.