FIGURE 4.

Glucose, octopine, and arginine levels following 1 h recovery from intense contractility. One of two paired mantle muscle sheets from cuttlefish (Sepia officinalis) was stimulated to contract for 30 s at 3 Hz simulating jetting behavior. (A,C,E) tissue concentrations after a 1 h period following contraction. (B,D) rate of uptake or release of metabolite into the bathing medium during the 1 h period following contraction. Open bars represent unstimulated preparations; stippled bars indicate preparations that were stimulated. Text below x-axis shows additions to media. For CHX and IAA additions, preparations were incubated for 10 min prior to stimulation, during stimulation, and throughout the post contraction incubation period. N = 6 under all conditions except tissue glucose in stimulated preparations incubated with CHX, tissue octopine in unstimulated preparations incubated with CHX, and media glucose in unstimulated preparations incubated with IAA where N = 5. Values with different letters are significantly different. ^∗Significant difference from zero (one sample t-test) for rate of glucose appearance in bathing medium. Box and whisker plots show the range and median value.