Basal and Induced Gene Expression of Relevant OA Genes in Response to IL-1β and TNF-α
(A) qRT-PCR analysis of IL6, IL8, IL1β, MMP13, and ADAMTS5 mRNA levels in non-treated cells and in response to stimulation by IL-1β and TNF-α in chondrocytes from three OA donors. Error bars represent a 95% confidence interval from technical triplicates. (B) Western blot analysis of IL-6 and IL-8 protein levels in non-treated and IL-1β and TNF-α-stimulated conditions in the same donors. β-actin (ACTB) was used as loading control.