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Validation of Increased Levels of miRNAs following Transfection
(A) qRT-PCR analysis of miR-140-5p, miR-140-3p, and miR-146a mRNA levels in chondrocytes from three donors after transfection. Error bars represent a 95% confidence interval from technical triplicates. (B) Western blot analysis of RALA and TRAF6 validated direct targets of miR-140-5p and miR-146a, respectively, in three donors. ACTB was used as loading control.
