Figure 4.

Validation of Proteomics Results by qRT-PCR and Western Blot Analysis

(A) GBRAP protein levels: ACTB was used as loading control. (B) GABARAP mRNA levels and (C) GBRAPI and -II after treatment with Bafilomycin A1. GAPDH was used as loading control. (D) CIR, STAT5A, RALA, and DHCR24 mRNA levels after overexpression of miR-140-5p in three donors. (E) LTOR5 and PDCD4 protein levels after overexpression of miR-140-3p in a fourth donor. (F) LAMTOR5 mRNA levels and (G) CIR, STAT2, BROX, and DDAH1 mRNA levels after overexpression of miR-140-3p in three donors. (H) RIPK2 protein levels, (I) RIPK2 mRNA levels, and (J) STAT2 and TP5313 mRNA levels after overexpression of miR-146a in three donors. ACTB was used as loading control for all western blots. Error bars from qRT-PCR represent a 95% confidence interval from technical triplicates.