Table 2.
Altered Proteins upon miR-140-3p Overexpression
| Protein | Protein Name | Fold Change | Biological Process | Predicted Targets |
|---|---|---|---|---|
| Upregulated by miR-140-3p | ||||
| ISAPP | RelA-associated inhibitor | INFa | inflammation, immune response, cell growth, apoptosis | |
| GILT | gamma-interferon-inducible lysosomal thiol reductase | INFa | ||
| CNPY4 | protein canopy homolog 4 | 2.8 | ||
| STAT3 | signal transducer and activator of transcription 3 | 2.1 | ||
| DDAH1 | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 | INFa | mitochondrial respiratory machinery, NOS/ROS regulation | |
| NDUS7 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial | 3.1 | ||
| PPIL3 DSS1 |
peptidyl-prolyl cis-trans isomerase-like 3 26S proteasome complex subunit DSS1 |
5.2 INFa |
proteasome, immunoproteasome, chaperones | |
| PSMG2 | proteasome assembly chaperone 2 | INFa | ||
| PSMD9 | 26S proteasome non-ATPase regulatory subunit 9 | INFa | ||
| ZFPL1 | zinc finger protein-like 1 | INFa | ER-protein, Golgi, vesicle trafficking | |
| COG3 | conserved oligomeric Golgi complex subunit 3 | INFa | ||
| VP37C | vacuolar protein sorting-associated protein 37C | 2.8 | ||
| ERLEC | endoplasmic reticulum lectin 1 | 2.0 | ||
| PDCD4 | programmed cell death protein 4 | 10.0 | tumor suppressor, apoptosis | |
| IBP4 | insulin-like growth factor-binding protein 4 | INFa | ||
| PP4R1 | serine/threonine-protein phosphatase 4 regulatory subunit 1 | INFa | chromatin, histone modifications, nuclear, mRNA export from the nucleus | |
| NUP93 | nuclear pore complex protein Nup93 | 7.2 | ||
| MEP50 | methylosome protein 50 | 5.1 | splisosome, transcription regulation, mRNA/DNA processing | |
| RNH2A | ribonuclease H2 subunit A | INFa | ||
| T2AG | transcription initiation factor IIA subunit 2 | INFa | ||
| MYOV2 | myeloma-overexpressed gene 2 protein | 2.0 | ||
| PININ | Pinin | 2.0 | ||
| PP12C | protein phosphatase 1 regulatory subunit 12C | INFa | scaffold protein, actin cytoskeleton | |
| NHRF1 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | 2.0 | ||
| FSTL1 | follistatin-related protein 1 | 2.2 | skeletal developement | |
| PCOC1 | procollagen C-endopeptidase enhancer 1 | 2.1 | glycoprotein that binds and drives enzymatic cleavage of type I procollagen | |
| Downegulated by miR-140-3p | ||||
| STA5A | signal transducer and activator of transcription 5A | −5.0 | inflammation, innate immunity | |
| C1R | complement C1r subcomponent | −2.0 | ||
| LTOR5 | regulator complex protein LAMTOR5 | −2.3 | autophagy | |
| MIC27 | MICOS complex subunit MIC27 | −5.0 | mitochondrial proteins and chaperopnes | |
| RT35 | 28S ribosomal protein S35, mitochondrial | −INFb | ||
| GPDM | glycerol-3-phosphate dehydrogenase, mitochondrial | −2.1 | ||
| CSTF1 | cleavage stimulation factor subunit 1 | −3.0 | mRNA processing | |
| BROX | BRO1 domain-containing protein | −3.0 | membrane bending | BROXc |
INF, infinity.
a
Only detected in miRNA transfected cells
b
Only detected in control transfected cells
c
Predicted target according to miRwalk: prediction based on 3′UTR