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. 2019 Aug 23;10:1822. doi: 10.3389/fmicb.2019.01822

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Copyright © 2019 Shrivastava, Shrivastava, Ninawe, Sharma, Kumar, Alam, Kanani, Sharma and Dash.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reproducibility of in-house indirect IgG ELISA. (A) Intra-assay variation (variation of replicates in one experiment) of IgG iELISA was performed with a panel of one from each low, medium, and high positive sample and three negative samples and five replicates within a plate. (B) Inter-assay-variation (variation (between experiments) performed on eight different days (days 1, 7, 10, 12, 17, 21, 30, and 40) in duplicates by the same operator. (C) Inter-operator variation (variation between experiments performed by different operators) and assay precision were conducted at different institutional laboratories and at FMD Centre, Gujarat, with a panel of seven positive and nine negative samples with different operators. Data interpretation was performed with Kruskal–Wallis and one-way ANOVA.)