Figure 2.

Coencapsulation of enzymes in the multicompartment protocell leads to an enhancement in the overall rate of reaction. (A) The enzyme cascade utilized. Glucose is oxidized by glucose oxidase to form gluconolactone and H₂O₂, which then diffuses to horseradish peroxidase, catalyzing the formation of fluorescent resorufin from amplex red. (B) Overall enzymatic rates of reaction as determined by bulk resorufin fluorescence in a plate reader. Coencapsulation of enzymes in polymersomes results in a rate increase compared to separately encapsulated enzymes. (C) Confocal images showing resorufin production over time inside a protocell containing coencapsulated GOx/HRP polymersomes (scale bars = 5 μm).