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The Hsp70 conjugate Hsp70-caaNBF is more potent than its parent Hsp70 in inhibiting in vitro α-synuclein aggregation. (a) Comparison of seeded α-synuclein aggregation assays in the presence of ATP alone as control (black), caaNBF (cyan), Hsp70 (purple), an equimolar mixture of control caaNBF and Hsp70 (green), and the NBF-modified chaperone Hsp70-caaNBF (red). (b) SDS-PAGE of the supernatant of the chaperone reactions after ultracentrifugation.
