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. Author manuscript; available in PMC: 2019 Aug 30.
Published in final edited form as: J Am Chem Soc. 2019 Aug 15;141(34):13582–13591. doi: 10.1021/jacs.9b06102

Figure 2 –

Figure 2 –

A) MftD was purified to homogeneity as determined by SDS-PAGE analysis. Lane 1 contains the Goldbio BLUEstain Protein Ladder standard, Lane 2 contains His6-purified MuMftD, and lane 3 contains His6-purified MsMftD. B) A UV-Vis spectral analysis of anaerobically prepared MftD indicates the presence of a reduced flavin (red). Upon exposure to air of the protein sample, the UV-Vis spectrum shifts considerably, indicating that the flavin is oxidized. C) High pressure liquid chromatography analysis of the flavin containing fraction of protein precipitate (red) indicates that MftD likely binds FMN when compared to retention times of flavin standards.