Figure 1.

A comparison of gene expression of Rca isoforms extracted from wheat leaves either under control conditions (22°C day/night) or after heat treatment. A, The spring wheat ‘Fielder’ harvested during the vegetative stage with heat treatment being 38°C/22°C for two diurnal cycles. B, The winter wheat ‘Mentor’ harvested during the flowering stage with heat treatment being 36°C/22°C for seven diurnal cycles. Gene expression analysis via reverse transcription quantitative PCR (qPCR) for cv Fielder used primers specific to the wheat TaRca1-β gene and TaRca2-α and TaRca2-β spliced variants, whereas for cv Mentor, primers were nonspecific for the TaRca2 spliced variants. Expression values are means ± sd of three or more biological replicates.