Figure 9.

Class II CIN TCP TFs control photoperiodic flowering. A, Representative images of the indicated genotypes showing their flowering phenotype under LD conditions. Three independent experiments were performed with each replica containing more than 30 plants for each line. Representative plants were photographed. B and C, Flowering phenotype of the indicated genotypes assessed by RLN (B) and DTF (C) under LD conditions. Values are means ± sd of ∼30 plants. Asterisks indicate significant differences; Student’s t test, **P < 0.01. The experiments were repeated at least three times with similar results. D, Expression of AP1, FUL, and LFY in the indicated genotypes. Two-week–old plants were harvested for total RNA extraction. Transcript levels of floral meristem identity genes in Col-0 plants were arbitrarily set to 1. ACTIN2 was used as an internal control. Values are means ± sd of three independent biological replicates. E and F, Expression of AP1 and TCP2/3/4/5/10/13/17/24 in the indicated genotypes. RNA was isolated from 2-week–old plants. ACTIN2 was used as an internal control. Values are means ± sd of three independent biological replicates. Asterisks indicate significant differences compared with that in wild type (WT); Student’s t test, **P < 0.01. G and H, Flowering phenotype of the indicated genotypes assessed by RLN (G) and DTF (H) under LD conditions. Values are means ± sd of ∼30 plants. Asterisks indicate significant differences compared with that in wild type; Student’s t test, **P < 0.01.