Table 2. Numbers of transcripts and metabolites that showed significant changes in M. truncatula monolignol pathway mutants compared with the wild type.
Microarray analysis was performed on 10d cse, comt, ccoaomt, and comt ccoaomt mutant plants, whereas RNA-seq analysis was performed on 10d hct and ccr1 mutant plants and on all whole stem samples. n = 6 (three replicates of each allele). ND, Not determined, as hct and comt ccoaomt mutant plants do not develop stem tissues; –, GC-MS analysis was not carried out on 10d ccr1 mutant plants.
| Genotype | 10d Seedlings | Mature Stem Tissue | ||||
|---|---|---|---|---|---|---|
| No. of Transcripts | No. of Compounds by GC-MS | No. of Compounds by UPLC-MS | No. of Transcripts | No. of Compounds by GC-MS | No. of Compounds by UPLC-MS | |
| hct | 6,750 | 89 | 33 | ND | ND | ND |
| cse | 2,837 | 52 | 20 | 9,659 | 83 | 119 |
| ccr1 | 1,272 | – | 14 | 14,286 | 70 | 111 |
| comt ccoaomt | 3,057 | 97 | 15 | ND | ND | ND |
| comt | 1,377 | 46 | 21 | 12,820 | 35 | 10 |
| ccoaomt | 777 | 49 | 22 | 10,753 | 47 | 1 |