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. 2019 Jul 16;181(1):353–366. doi: 10.1104/pp.19.00496

Figure 3.

Figure 3.

Expression of pp2c.d5M328K in Arabidopsis confers dramatic cell expansion defects. A, Twenty-four-day-old plants. Three independent pp2c.d5M328K-GFP lines are shown. Bar = 1 cm. B, Examination of AHA Thr-947 phosphorylation in 7-d-old light-grown seedlings by GST-14-3-3 far-western assay. PP2C.D5-GFP and pp2c.d5M328K-GFP protein expression levels are shown in the bottom image. C, Leaf blade area of 24-d-old plants. Values represent means (n = 30, three leaf blades per plant) ± sd. D, Seven-day-old light-grown seedlings. Bar = 1 mm. E, Hypocotyl length of 7-d-old light-grown seedlings. Error bars indicate sd (n = 20). F, Mean hypocotyl epidermal cell length of 7-d-old light-grown seedlings. The apical-most five cells from 10 seedlings were measured (n = 50). Error bars indicate sd. G, Five-day-old etiolated seedlings. Bar = 2 mm. H, Seven-week-old plants. Bar = 5 cm. I, Plant height of 7-week-old plants. Error bars indicate sd (n = 10). Different letters above bars indicate significant differences (P < 0.05) when analyzed by one-way ANOVA with Tukey’s honestly significant difference (HSD) test. In D and G, images are composites where individual images were cropped and digitally extracted for comparison.