Figure 5.

pp2c.d5^M328K is partially suppressed in ost2-2 aha2-5. A, Examination of AHA Thr-947 phosphorylation in 7-d-old light-grown seedlings by GST-14-3-3 far-western gel assay. B, Immunoblot examining pp2c.d5^M328K-GFP protein expression level in 7-d-old light-grown seedlings. SEC12 is shown as a loading control. C, Twenty-four-day-old plants. The left image shows a comparison between ost2-2 aha2-5 with or without the pp2c.d5^M328K-GFP transgene and pp2c.d5^M328K-GFP in the pp2c.d5 mutant background as a reference. The right image shows two independent lines of ost2-2 aha2-5[pp2c.d5^M328K-GFP] after backcrossing the transgene into the pp2c.d5 AHA1 AHA2 background. Bar = 1 cm. D, Leaf blade area of 25-d-old plants. Values represent means (n = 30, three leaf blades per plant) ± sd. E, Seven-day-old light-grown seedlings. Bar = 1 mm. F, Five-day-old etiolated seedlings. Bar = 2 mm. G, Mean hypocotyl length of 7-d-old light-grown seedlings. Error bars indicate sd (n = 20). H, Mean hypocotyl length of 5-d-old etiolated seedlings. Error bars indicate sd (n = 20). I, Flowers. The bottom images show flowers with sepals and petals removed to expose carpels and stamens. Bars = 1 mm. J, Seven-week-old plants. Bar = 5 cm. K, Plant height of 7-week-old plants. Error bars indicate sd (n = 10). L, Inflorescence stems showing siliques. White asterisks indicate undeveloped siliques in ost2-2 aha2-5 double mutant and ost2-2 aha2-5 [pp2c.d5^M328K-GFP] independent lines. Bar = 5 cm. M, Percentage of developed and undeveloped siliques. Error bars indicate sd (n = 200, 20 siliques per plant). Different letters above bars indicate significant difference (P < 0.05) when analyzed by one-way ANOVA with Tukey’s HSD test. In C, E, F, and I, images are composites where individual images were cropped and digitally extracted for comparison.