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. 2019 Jul 2;181(1):221–235. doi: 10.1104/pp.19.00082

Figure 10.

Figure 10.

Interactions among rice ZMM proteins. A, Analysis of interactions between OsSHOC1 and ZMM proteins using the Y2H assay. B, Analysis of interactions between ZMM proteins using the Y2H assay. Full-length OsSHOC1, OsPTD, and ZMM protein coding sequences were constructed into both pGADT7 (Gal4AD) and PGBKT7 (Gal4BD) vectors. Selective-medium -LT (SD-Leu/-Trp), and -LTHA (SD-Leu/-Trp/-His/-Ade) with X-α-gal, were used to test interactions in the Y2H system. C, Diagram summarizing the network of interactions among ZMM proteins identified by Y2H system. Two-way arrows indicate interactions between rice ZMM proteins identified in this study (black) and those in previous reports (red). D and E, Y3H analysis among OsSHOC1, OsPTD, OsHEI10, and OsZIP4. Full-length OsSHOC1 and OsPTD protein coding sequences were constructed into pGADT7 vectors, and OsZIP4, OsHEI10, OsSHOC1, and OsPTD were separately cloned into multiple cloning sites I (MCSI) and II (MCSII) of pBridge vectors. Yeast cells were grown on -LTM (SD-Leu/-Trp/-Met) selection agar plates or -LTHAM (SD-Leu/-Trp/-His/-Ade/-Met) agar plates with X-α-gal for interaction detection.