Immunodetection analysis of OsSHOC1 and OsPTD (magenta) in the wild type and the oszip4 and oshei10 mutants. A, OsSHOC1 foci in wild-type, oshei10, and oszip4 zygotene meiocytes. B, OsPTD foci in wild-type, oshei10, and oszip4 zygotene meiocytes. C, Statistical analysis of numbers of OsSHOC1 foci in wild-type (n = 16), oshei10 (n = 30), and oszip4 (n = 43) zygotene cells. D, Statistical analysis of numbers of OsPTD foci in wild-type (n = 12), oshei10 (n = 35), and oszip4 (n = 40) zygotene cells. All values represent the mean ± sd. The asterisks indicate statistical significance between the wild type and the mutants, as determined by one-way ANOVA test with post hoc contrasts by the Newman-Keuls multiple-comparisons test (****P < 0.0001). Meiotic chromosomes are indicated by anti-OsREC8 antibody (green). Bars = 2 μm.